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Image Search Results
Journal: Cell chemical biology
Article Title: Probing Exchange Units for Combining Iterative and Linear Fungal Nonribosomal Peptide Synthetases.
doi: 10.1016/j.chembiol.2019.08.005
Figure Lengend Snippet: Figure 2. Exchange of C-A(Mt)-T Units in CDP Synthetases Overview of the in vivo CDP production of wild-type and artificial synthetases in E. coli based on EnSYN (blue) and BaSYN (green) with swapped full module 2 (xM2), as observed by MALDI-TOF-MS. Hybrid units were taken from CySYN modules 2 (N-Me-L-Leu; light pink), 4 (N-Me-L-Val; pink), 7 (N-Me-Gly; dark pink), and 10 (N-Me-L-Leu; magenta). The specificities of the cognate or hybrid C2 domain at the don- and acc-sites are indicated next to each synthetase in black (intrinsically respected) and red (disrespected in the hybrid system). See also Figures S2–S4.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Fungal Strains E. coli DH5a (K12) Datsenko and Wanner, 2000 N/A E. coli BL21 gold (DE3)_npgA Steiniger et al., 2017 N/A Beauveria bassiana ATCC ATCC7159 Fusarium
Techniques: In Vivo
Journal: Cell chemical biology
Article Title: Probing Exchange Units for Combining Iterative and Linear Fungal Nonribosomal Peptide Synthetases.
doi: 10.1016/j.chembiol.2019.08.005
Figure Lengend Snippet: Figure 3. Exchange of A(Mt)-T Units in CDP Synthetases Overview of the in vivo CDP production of wild-type and artificial synthetases in E. coli based on EnSYN (blue) and BaSYN (green) with swapped A(Mt)-T part of module 2 (x(AMtT)2), as observed by MALDI-TOF-MS. Hybrid units were taken from CySYN modules 2 (N-Me-L-Leu; light pink), 4 (N-Me-L-Val; pink), 7 (N-Me-Gly; dark pink), and 10 (N-Me-L-Leu; magenta). The specificities of the cognate or hybrid C2 domain at the don- and acc-sites are indicated next to each synthetase in black (intrinsically respected), green (respected in the hybrid system), or red (disrespected). See also Figures S2–S4.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Fungal Strains E. coli DH5a (K12) Datsenko and Wanner, 2000 N/A E. coli BL21 gold (DE3)_npgA Steiniger et al., 2017 N/A Beauveria bassiana ATCC ATCC7159 Fusarium
Techniques: In Vivo
Journal: Cell chemical biology
Article Title: Probing Exchange Units for Combining Iterative and Linear Fungal Nonribosomal Peptide Synthetases.
doi: 10.1016/j.chembiol.2019.08.005
Figure Lengend Snippet: Figure 4. Exchange of CCTD-A(Mt)-T Units in CDP Synthetases Overview of the in vivo CDP production of wild-type and artificial synthetases in E. coli based on EnSYN (blue) and BaSYN (green) with swapped CCTD-A(Mt)-T part of module 2 (xCTDM2), as observed by MALDI-TOF-MS. Hybrid units were taken from CySYN module 2 (N-Me-L-Leu; light pink), 4 (N-Me-L-Val; pink), 7 (N-Me- Gly; dark pink) and 10 (N-Me-L-Leu; magenta). The specificities of the cognate or hybrid C2 domain at the don- and acc-sites are indicated next to each syn- thetase in black (intrinsically respected) or gray (might arise from EnSYN- or CySYN-derived C subdomains). See also Figures S2–S4.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Fungal Strains E. coli DH5a (K12) Datsenko and Wanner, 2000 N/A E. coli BL21 gold (DE3)_npgA Steiniger et al., 2017 N/A Beauveria bassiana ATCC ATCC7159 Fusarium
Techniques: In Vivo, Derivative Assay